Nontoxic nanoparticle delivers and tracks drugs
The battlefield of the future may react differently to combat injuries, providing instant treatment to wounded soldiers even before a medic reaches their side. Impossible? Not if researchers at universities on opposite sides of the country succeed in creating a “field hospital on a chip” - a system worn by every soldier that would detect
Full Post: Researchers develop ‘field hospital on a chip’
A nontoxic nanoparticle developed by Penn State researchers is proving to be an all-around effective delivery system for both therapeutic drugs and the fluorescent dyes that can track their delivery.
In a recent online issue of Nano Letters, an interdisciplinary group of materials scientists, chemists, bioengineers, physicists, and pharmacologists show that calcium phosphate particles ranging in size from 20 to 50 nanometers will successfully enter cells and dissolve harmlessly, releasing their cargo of drugs or dye.
Peter Butler, associate professor of bioengineering, and his students used high-speed lasers to measure the size of fluorescent dye-containing particles from their diffusion in solution.
“We use a technique called time correlated single photon counting,” Butler says. “This uses pulses of laser light to read the time, on the order of nanoseconds, that molecules fluoresce.”
With this method, his group was able to measure the size of the particles and their dispersion in solution, in this case a phosphate-buffered saline that is used as a simple model for blood.
“What we did in this study was to change the original neutral pH of the solution, which is similar to blood, to a more acidic environment, such as around solid tumors and in the parts of the cell that collect the nanoparticles-containing fluid immediately outside the cell membrane and bring it into the cell. When we lower the pH, the acidic environment dissolves the calcium phosphate particle,” he adds.
“We can see that the size of the particles gets very small, essentially down to the size of the free dye that was inside the particles. That gives us evidence that this pH change can be used as a mechanism to release any drug that is encapsulated in the particle,” Butler explains.
Although the primary use envisioned for these particles is for targeted cancer therapy, Butler’s group is interested in their ability to deliver various drugs that have been shown to inhibit cell growth associated with vascular disease.
Several drugs have been shown in cultures to be promising for reducing hardening of the arteries and narrowing of blood vessels after balloon angioplasty. The problem has been in delivering any of these drugs to a target, Butler says.
Ceramide, a chemotherapeutic molecule that initiates cell death in cancer cells, has the ability to slow growth in healthy cells.
Mark Kester, professor of pharmacology, and Jong Yun, associate professor of pharmacology, both at Penn State College of Medicine, have optimized ceramide for both cancer and vascular disease.
Their groups found that by using human vascular smooth muscle cells in vitro, ceramide encapsulated in calcium phosphate nanoparticles reduced growth of muscle cells by up to 80 percent at a dose 25 times lower than ceramide administered freely, without damaging the cells.
The calcium phosphate nanoparticles were developed by James Adair, professor of materials science and engineering, and his students. The nanoparticles have several benefits other drug delivery systems do not, according to lead author Thomas Morgan, graduate student in chemistry.
Unlike quantum dots, which are composed of toxic metals, calcium phosphate is a safe, naturally occurring mineral that already is present in substantial amounts in the bloodstream.
“What distinguishes our method are smaller particles (for uptake into cells), no agglomeration (particles are dispersed evenly in solution), and that we put drugs or dyes inside the particle where they are protected, rather than on the surface,” says Morgan. “For reasons we don’t yet understand, fluorescent dyes encapsulated within our nanoparticles are four times brighter than free dyes.
“Drugs and dyes are expensive,” he continues, “but an advantage of encapsulation is that you need much less of them. We can make high concentrations in the lab, and dilute them way down and still be effective. We even believe we can combine drug and dye delivery for simultaneous tracking and treatment. That’s one of the things we are currently working on.”
A tiny particle syringe composed of polymer layers and nanoparticles may provide drug delivery that targets diseased cells without harming the rest of the body, according to a team of chemical engineers. This delivery system could be robust and flexible enough to deliver a variety of substances. “People probably fear the effects of some
Full Post: Tiny particle syringe for drug delivery
Using tiny gold particles and infrared light, MIT researchers have developed a drug-delivery system that allows multiple drugs to be released in a controlled fashion. Such a system could one day be used to provide more control when battling diseases commonly treated with more than one drug, according to the researchers. “With a lot of
Full Post: Gold particles and infrared light for controlled drug delivery
Antibodies that target epidermal growth factor receptor (EGFR) have proven themselves as potent anticancer drugs. Now, a team of investigators led by Shuming Nie, Ph.D., and Lily Yang, Ph.D., both at the Emory University School of Medicine and members of the Emory-Georgia Tech Nanotechnology Center for Personalized and Predictive Oncology, is aiming to capitalize on
Full Post: Artificial antibody delivers nanoparticles to tumors
Healthy adults with higher levels of phosphate in the blood are more likely to have increased levels of calcium in the coronary arteries-a key indicator of atherosclerosis and future cardiovascular disease risk, reports a study in the February 2009 issue of the Journal of the American Society of Nephrology (JASN). “Phosphate level may represent a
Full Post: Higher levels of blood phosphate may be a previously unidentified and modifiable cardiovascular risk factor
Researchers at the University of Delaware have provided what is believed to be the first experimental evidence that plants can take up nanoparticles and accumulate them in their tissues. The laboratory study, which involved pumpkin plants, indicates a possible pathway for nanoparticles to enter the food chain. The research also reveals a new experimental
Full Post: Plants shown to accumulate nanoparticles in tissues